HOT-COFI TECHNOLOGY

The Hot-Colony Filtration ("Hot-CoFi") is DotBio’s proprietary stabilization technology. The Hot-CoFi technology allows us to improve the stability and solubility of our DotBodies. This leads to better developability and safety profile of the molecules we generate.

This proprietary technology relies on a filtration step to identify more stable variants of a target protein. It is a very robust biophysical technique performed at the bacteria colony level. It has been thoroughly validated against a diverse set of protein targets.

Image of the results obtained with the Hot-CoFi blot. Each spot represents a bacterial colony expressing a variant of the target protein. The spots with darker color are the variants with higher stability.

Aggregation-propensity leads to manufacturing issues and increased risk of adverse immune reactions.

Our stable DotBody technology has been optimized by Hot-CoFi, to yield molecules with favorable properties for therapeutic development.